Application

Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.

Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).

Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.

Immunoprecipitation: 1:1,000-1:10,000

ELISA : 1:1,000

Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)

Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)

Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)

Structure-function of ACE (Danilov et al., 1994)

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9 detects level of Angiotensin Converting Enzyme & has been published & validated for use in ELISA, IP, WB, IC, IH.

Research Sub CategoryHormones & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Angiotensin-converting enzyme from human lung

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in PBS, pH 7.4, 150 mM NaCl containing 0.05% sodium azide.

Specificity

Angiotensin-converting enzyme [ACE] (N-terminal), no reactivity to testicular/germinal form of human ACE (90kDa isoform)

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.